CHEM Seminar: "Capturing Organelle Dynamics with Mass Spectrometry-based Omics Strategies"
Tuesday, September 26, 2023
1110 Jeong H. Kim Engineering Building
Host: Peter Nemes (email@example.com)
Speaker: Ling Hao (George Washington University)
Title: "Capturing Organelle Dynamics with Mass Spectrometry-based Omics Strategies"
Abstract: Lysosomes and mitochondria are membrane-bound organelles in the cell that are responsible for trash-disposal and ATP production, respectively. Lysosomal and mitochondrial dysfunctions have been linked to numerous human diseases, such as neurodegeneration, cancer, and cardiovascular diseases. These organelles are highly dynamic and frequently interact with other cellular components in a transient fashion, which are difficult to capture with traditional immunoprecipitation methods. Here, I will describe our recent efforts in developing proximity labeling, organelle isolation, and dynamic SILAC proteomic methods to characterize sub-organelle microenvironment and protein dynamics in human stem cell-derived neurons. I will also demonstrate how we implement mass spectrometry-based multi-omics strategies to understand cell biology and molecular mechanisms of brain diseases.
Bio: Dr. Ling Hao is an Assistant Professor in the Chemistry Department at the George Washington University. She received her PhD from the University of Wisconsin-Madison followed by a postdoctoral training at the National Institutes of Health. Since joining GW in 2019, she has received the NSF CAREER Award and an NIH R01 grant to support her research in developing mass spectrometry-based omics methods to study brain diseases. She was also recognized as an Emerging Investigator by the Journal of American Society for Mass Spectrometry (JASMS) in 2021 and a Rising Star Award by the Human Proteome Organization (HUPO) World Congress in 2022. Most recently, she received the Cottrell Scholar Award from the Research Corporation for Science Advancement for her dedication in science education.